FastQCFastQC Report
Thu 8 Feb 2024
control_201_untreated_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_201_untreated_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31711086
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTGTGC3503081.104686228658331TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGGTGC2693840.8494947161380724TruSeq Adapter, Index 12 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGGTGG2573880.8116656742692445TruSeq Adapter, Index 12 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTGTGG2294720.723633369100005TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2019060.6367047788902594TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGG852180.2687325183375934TruSeq Adapter, Index 12 (97% over 49bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT692020.21822652179114901No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGC618810.19513995830984784TruSeq Adapter, Index 12 (98% over 50bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC480130.1514076181433837No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGG359940.11350604643436052TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGTGTGC353490.11147205743757876TruSeq Adapter, Index 12 (97% over 46bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA352690.11121977973255158No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG335730.10587149238597506No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT333100.10504212943069816No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC327550.10329195285207197TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGG318130.10032138287537676TruSeq Adapter, Index 12 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2565400.073.155689
AGAGCAC2593550.072.3708048
CGGAAGA2651350.070.370844
ATCGGAA2691600.069.540612
AAGAGCA2716300.069.318787
TCGGAAG2715900.068.719153
GATCGGA2729550.068.563461
GAAGAGC2758650.068.056756
GGAAGAG2981950.062.9779055
AATCTCG1895150.045.12251738-39
TCGTATG332100.044.57007242-43
GTAATCT2165650.043.51371836-37
ATCTCGT1124700.043.03938740-41
TCGGGTG637850.043.03294842-43
TATGCCG252950.043.01804746-47
CCTTGTA2209650.042.58931432-33
TCTCGTG706300.042.4879840-41
TAATCTC1997500.042.32811738-39
CTCGTGT702350.042.2234742-43
TTGTAAT2240800.042.13170634-35
Produced by FastQC (version 0.11.4)