FastQCFastQC Report
Thu 8 Feb 2024
control_201_treated_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_201_treated_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30381170
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21903457.209547887721243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG49500.031.570483
CAATCGC60500.026.2228224
AATCGCC77250.021.0288735
TGGCGCG125200.017.7934043
ATCAATC100300.016.4804692
GATCAAT109450.014.6687171
ATCGCCC122400.014.0480336
GGCGCGT217600.012.3114334
GTGGCGC194950.011.8170672
GGTCGAC23350.011.5951485
CTACTCG281000.011.4100056
TACTCGG286400.011.2777977
CGCGTGC236750.011.1751556
CGATCAA31550.010.6893231
TCGCCCC164850.010.4305697
GCGTGCC233600.010.3701667
GGGTCGA26150.010.1719624
ACTCGGG332000.09.9148398
CGAGAAT76250.09.8425691
CGGAGGG121600.09.6874481
Produced by FastQC (version 0.11.4)