FastQCFastQC Report
Thu 8 Feb 2024
control_201_treated_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecontrol_201_treated_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30381170
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC17201065.661750353919879TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTGGTATGC1883500.6199563742936826TruSeq Adapter, Index 11 (98% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT305780.10064786840006491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2526800.079.124479
ATCGGAA2532400.078.8424152
AGAGCAC2540700.078.7420358
CGGAAGA2533200.078.531094
GATCGGA2546550.078.413411
TCGGAAG2583750.077.047223
AAGAGCA2640700.075.966887
GAAGAGC2667850.074.960726
GGAAGAG2870900.069.6490555
TCGTATG1902200.046.36076742-43
TATGCCG2096200.045.7564846-47
TCTCGTA1936600.045.6683840-41
CGGCTAC2196650.045.56998432-33
CGTATGC1907150.045.48633244-45
ATGCCGT2121200.045.4510446-47
GTATGCC2136800.045.4313144-45
ACGGCTA2197650.045.12592732-33
ATCTCGT1980100.044.86886240-41
GCCGTCT2075650.044.82715248-49
CATCTCG2031650.044.54340738-39
Produced by FastQC (version 0.11.4)