FastQCFastQC Report
Thu 9 May 2024
SRR576524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24323161
Sequences flagged as poor quality0
Sequence length100
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTTGCGCAGTGGCAGTATCGTAGCCAATGAGGTTTATCCGAGGCGC2600721.0692360256958378No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC1670900.6869584097231441No Hit
TGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGT1607650.6609543882885945No Hit
TGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA1508120.6200345423853422No Hit
TCCAGCTTTGCGCAGTGGCAGTATCGTAGCCAATGAGGTTTATCCGAGGC1256720.5166762658850138No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCG1122440.46146962559677174No Hit
TAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCTC1033310.4248255397396744No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCT958200.39394550732941336No Hit
TAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAG810820.3333530539061103No Hit
TAGCTTTGCGCAGTGGCAGTATCGTAGCCAATGAGGTCTATCCGAGGCGC778170.31992963414582504No Hit
TCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCC777570.31968295568162375No Hit
TGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCC726290.2986001696078894No Hit
TAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAAC689820.2836062302921894No Hit
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAG656300.2698251267588123No Hit
TAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGC568540.23374429006164124No Hit
TGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGT565370.23244100550911126No Hit
TGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCC541640.22268487224995137No Hit
TAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTG484080.19902018491757714No Hit
TAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGT441960.1817033567306486No Hit
TGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGC441750.18161701926817816No Hit
TAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCG426710.1754336124321999No Hit
TGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA412010.16938999005926902No Hit
TCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGA403900.16605571948481532No Hit
TCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGA383250.15756586900855526No Hit
TCAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGAC354140.14559785218705742No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATT344200.14151121229678987No Hit
TATTAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTC343210.14110419283085782No Hit
TACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAG337120.138600406419215No Hit
TCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGG329350.13540592030780868No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCA325360.1337655085208703No Hit
TACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAAC318230.130834146104612No Hit
TCAGCTTTGCGCAGTGGCAGTATCGTAGCCAATGAGGTTTATCCGAGGCG309340.12717919352669663No Hit
TAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAG307540.12643915813409284No Hit
TAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTC306790.12613081005384127No Hit
TGGCAGCACATATACTAAAATTGGAACGATACAGAGAAGATTAGCATGGC293160.12052709760873596No Hit
TAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT282640.11620200186974053No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGC279230.11480004593153004No Hit
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGC271690.11170011989806754No Hit
TGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCA263110.1081726178599895No Hit
TAGCACATATACTAAAATTGGAACGATACAGAGAAGATTAGCATGGCCCC261500.10751069731438279No Hit
TGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACG255860.10519191975089093No Hit
TGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGC255250.10494112997895298No Hit
TAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACT249990.10277858210945526No Hit
TAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAG243900.10027479569781246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTT467650.090.185581
GTCTACG291650.087.047432
TAGTCAG428500.085.39221
TCTACGG306150.083.3085563
TGTCTAC310950.082.010681
CGGCCAT326850.078.312947
TACGGCC328250.077.907385
ACGGCCA331350.077.079276
GCCATAC330550.076.895069
CTACGGC334600.076.760214
GGCCATA335250.076.517638
TGTCAGC368350.074.527881
TAGCAGC231300.067.687351
TATCGCT29750.065.10861
TAGCGTG39050.064.567841
TACGGGT40200.063.8930971
TTGCGCA661850.063.565256
TAGCACT95950.063.263671
TTTGCGC669250.062.82735
TAGTGAA118200.062.4791341
Produced by FastQC (version 0.11.4)