FastQCFastQC Report
Thu 9 May 2024
SRR576523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576523_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences143338267
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1580310.11025039112549058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCTA1753900.024.492622
AGCTTCT1786300.024.4729541
ACCTCAG1855050.024.0757065
AGACGTG1581450.024.06806815
GACCTCA1870900.023.9503484
ATCAGAC1712050.023.80749112
CTCAGAT1848250.023.7177287
GACGTGG1415800.023.7171616
CCTCAGA1846850.023.714576
CGACCTC1915650.023.5867163
TCAGACG1697850.023.41938813
CAGACGT1709250.023.07379514
TCTAGGA970550.023.0683525
GTGGCGA1303750.022.9743819
ACTGAAA243550.022.78199627
CTAAAGG751350.022.712856
CAGATCA1957200.022.416439
AGATCAG1967800.022.31482710
TTCTAGG1011400.022.2263684
TGGCGAC1325050.022.04907220
Produced by FastQC (version 0.11.4)