FastQCFastQC Report
Thu 9 May 2024
SRR576522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576522_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences186384729
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3486440.18705609728359238No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2023360.10855824996263508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT516100.012.9107392
CTTAACA312150.012.10464911
TTAACAC308650.011.6036212
TAAGGTT2756550.011.43853529
ATTCCAA2855750.011.42644829
CGGAACC2824700.011.38145829
GCTTAAC334250.011.11334410
TAGCTTA397200.011.08723458
TTCCAAT1975100.010.96712129
AAGGTTA1930050.010.92831829
GGAACCG2105950.010.89219729
CCTTGGC12306450.010.86693929
AGACGTG563900.010.7566615
ATAAAAA655000.010.70513629
CAGACGT584200.010.70302614
AGCTTAA369750.010.5792779
GACGTGG527750.010.35039916
GCCTTGG18856450.010.25837929
TATAAAA981950.010.23148529
CCTCAGA647400.010.1009696
Produced by FastQC (version 0.11.4)