FastQCFastQC Report
Thu 9 May 2024
SRR576521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576521_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences148441452
Sequences flagged as poor quality0
Sequence length50
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAAG1019750.037.3534971
TCAGTTG943000.035.27081712
GTAGAGC1123250.034.81996519
GTTGGTA764500.034.77505515
CAAGGCT754950.033.654127
TTGGTAG923800.033.36881316
GCTCAGT1398850.033.25463510
CAGTTGG1005300.033.2296313
CCGTACT3393800.033.1277520
AGTTGGT996800.032.734914
CAAGTGA1157450.032.636434
TCAAGTG1169100.032.4302833
TAGCTCA1793000.032.4200448
GTAGCTC751600.032.4056137
CTCAGTT1037850.032.314211
TGTAGCT475350.032.1358036
TAGTGGT741350.031.56147613
AGCACGT896700.031.56065221
TTAGTTA1007350.031.23165912
AGCCGTA3603350.030.84101318
Produced by FastQC (version 0.11.4)