FastQCFastQC Report
Thu 9 May 2024
SRR576520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576520_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences9447811
Sequences flagged as poor quality0
Sequence length35
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGG26550.026.0247972
TTCGGCA25200.025.306394
CGCTTCG29900.024.842471
CTTCGGC26550.024.2357123
AACTGTT13850.021.30785229
AACTCAA39650.020.6789929
TCGGCAG27850.020.4772035
GGTCTGG385550.020.31232829
ATGCCTG15350.019.8258971
CTTGAGT110500.019.61592529
TCCAGAC188600.019.5479429
GAACTCA139700.019.41818429
GCAAAGG12900.019.2308061
GGTGTTG35200.018.894863
CCAGACC56700.018.79378929
CTTGACA28300.018.4930929
CGGGTCT8450.018.2635781
AGGTCTG1094850.018.2561829
CTTGTCA16650.018.2484329
GGGTGTT36250.018.185472
Produced by FastQC (version 0.11.4)