FastQCFastQC Report
Thu 9 May 2024
SRR576519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576519_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences2592379
Sequences flagged as poor quality0
Sequence length35
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCGGCAGCACCGCCTTGGCCGTACAGCAGAC34690.1338153101841976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGC10000.026.3806343
TTCGGCA10050.026.1056674
GCTTCGG10600.025.9813442
CGCTTCG12350.023.003981
ATGTGGC7400.022.3299141
ACGAAAG15650.021.7654381
AACCCGG13300.021.687791
TCGGCAG11900.021.6817995
AAACGAG1350.020.4001661
CAAAGCA15350.019.9245241
CGTACGG7950.019.1445187
AGGCGTC9850.018.9831091
AGTACCT2550.018.7580471
CAACCCG4200.018.6362421
GTACGGA8200.018.5615638
GTGGCGT8500.018.416993
TGGCGTA8750.017.725484
CTGGGAA8200.017.6766531
ACTCGGC7900.017.6140021
GTCCAGT7800.017.0968255
Produced by FastQC (version 0.11.4)