FastQCFastQC Report
Thu 9 May 2024
SRR576518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576518_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences88400455
Sequences flagged as poor quality0
Sequence length35
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGACCCCTCGCCTTGGCCGTACAGCAGCCG1012660.11455370902785512No Hit
CCCCCGCCGGTCGCCTTGGCCGTACAGCAGCCGAT976590.11047341328729585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGAG628950.021.97983223
TGAGAGA508950.021.50807224
GATGAGA672950.019.5485122
GACATCA103100.018.6953471
AGAGAAT245250.018.60826726
GAGAGAA307650.018.29284525
GAATCTC100800.017.418829
ACGAAAG101400.017.222241
CCTCCCG814650.016.9660721
AGAATCT134050.016.90855228
CTGGGAA146200.016.9012011
CAGCTAG221500.016.69241329
AACCCGG122350.016.6896651
AGCCGAT7485950.016.48834229
GCCGCGG1124000.016.3519252
GAGAATC155300.016.23895827
CGATGAG829000.016.23409821
TCGGCTA1581900.015.99323729
GCCAAGG98300.015.8929981
CCGCGGG1002000.015.7659563
Produced by FastQC (version 0.11.4)