FastQCFastQC Report
Thu 9 May 2024
SRR576517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576517_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences27692385
Sequences flagged as poor quality0
Sequence length35
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGG118400.025.837732
TTCGGCA110250.025.7366564
CTTCGGC113500.025.443753
CGCTTCG130900.025.3625091
TCGGCAG121000.022.9867445
CAGCCGA1290000.021.31388329
TGATTAG117800.018.9022529
ACTAATC90850.017.31021129
AACCCGG61050.017.1343481
AAATTCG45550.016.819821
GGGTGTT96550.016.6645182
AATTCGT43250.016.4486792
CGCTAAT93550.016.4066629
GCAGCCG3747850.016.40276729
CGATTAG29500.016.30819929
TCGGCTA93100.016.22056429
CGGCAGC175250.016.143426
AGCCGAT346500.016.08232729
GTGTTGA96750.016.02144
GGTGTTG100800.015.9927273
Produced by FastQC (version 0.11.4)