FastQCFastQC Report
Thu 9 May 2024
SRR576516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576516_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences26418123
Sequences flagged as poor quality0
Sequence length35
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGGGTGTGAGAGAACAGA1047740.396598955951564No Hit
CGCCTTGGCCGTACAGCAGGGTGTGAGAGAGCAGA572430.2166807990105883No Hit
CGCCTTGGCCGTACAGCAGGGTGTGAGAGAGTGAG557720.21111265172018465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAGA1126350.026.4356924
GAACAGA120850.026.1992729
GAATGAG115750.026.08748429
AGAACAG191900.026.0114528
AGAGAAT261000.026.01089126
GAGAGAA680000.025.7646425
AGAGAAC306450.025.72703726
AGAATGA168900.025.64459228
GAGAATG182500.025.64108727
GAGAACA212550.025.47710627
GTGAGAG1603600.025.1210523
AGAGAGT177500.024.96679126
TGTGAGA1638850.024.79713222
TGAGAGT239650.024.55408724
GAGAGAG442500.023.8758425
GTGTGAG1870750.023.24413721
GACATCA40800.023.160061
AGAGAGC200450.023.05629726
AGAACTC63100.022.97297528
AGAGTGA102350.022.48518628
Produced by FastQC (version 0.11.4)