FastQCFastQC Report
Thu 9 May 2024
SRR576515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576515_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences86640039
Sequences flagged as poor quality0
Sequence length35
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12680591.4635946782064584No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9360861.0804311849397945No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7093480.8187300100361219No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6718710.7754740276605832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAAA2323950.022.58614329
CTCCTTT1407550.021.99614729
TCTTCCC1488750.021.52716829
AGAAGGG5705450.021.49906729
TCTTTTT238150.020.60830929
AGAAAAA330900.019.90605529
GAAGGGG1540900.019.3969529
AGAATTT301000.018.85787629
CGAGGAA1628450.018.5039629
TTCGGCA94400.018.3285884
CTTCGGC97900.018.1478923
AAAGGAT138100.018.0719873
GAGGTTT334250.017.96403129
TCCTTTT411750.017.94809229
GCTTCGG106900.017.8233152
TCTTAAA188650.017.61586429
TCTTGGG252150.017.51133729
GGTGTTG326900.017.42013
CTCTTTT159000.017.30165129
GTGTTGA321850.017.2027324
Produced by FastQC (version 0.11.4)