FastQCFastQC Report
Thu 9 May 2024
SRR576514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR576514_1.fastq.gz
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences9636262
Sequences flagged as poor quality0
Sequence length35
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGGGGCTTAGAGAATCTC114830.11916446439501126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAAGG59350.026.4923921
GCCAAGT16350.026.1466711
TAGAGAA105450.026.1350925
TTAGAGA132200.026.102124
CATAGTG18350.025.3504492
AGAGAAT59100.025.25788326
TGCAGCC45700.024.8031437
AGGCTGC11700.024.5247763
AGAGAAC26850.024.47501626
CCCTGCA28700.024.2415124
CTGCAGC24550.024.1482546
CCTGCAG24400.023.8209695
AGTGCAG19200.023.7801275
CTAGTGC12300.023.6819083
TGCAGTC19000.023.344477
CCTAGTG10250.023.1865792
TAGTGCC15750.023.0990164
CTGCATG11700.023.0431046
TGCTGTA16800.022.9448131
TAGTGCA12450.022.934924
Produced by FastQC (version 0.11.4)