SRR576513_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR576513_1.fastq.gz
File type	Colorspace converted to bases
Encoding	Sanger / Illumina 1.9
Total Sequences	22698550
Sequences flagged as poor quality	0
Sequence length	35
%GC	59

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACATCACTTACGCCGCCTTGGCCGTACAGCAGA	611423	2.6936654544012724	No Hit
GGACATCACTTACGCCTTGGCCGTACAGCAGACGG	400938	1.766359525167907	No Hit
GGAGGAGAAGAAACCGCCTTGGCCGTACAGCAGAC	378650	1.668168231010351	No Hit
GGACATCACATACGCCGCCTTGGCCGTACAGCAGA	203462	0.89636562687925	No Hit
GGACATCACGTACGCCGCCTTGGCCGTACAGCAGA	94666	0.4170574772397356	No Hit
GGACATCACTAACGCCGCCTTGGCCGTACAGCAGA	66247	0.2918556471668895	No Hit
GGAGGAAAAGAAACCGCCTTGGCCGTACAGCAGAC	63232	0.27857286038094947	No Hit
AAAGCATCGCGAAGGCCCGCCGCCTTGGCCGTACA	62518	0.2754272850027865	No Hit
AAAGCATCGCGAAGACCCGCCGCCTTGGCCGTACA	48645	0.2143088435164361	No Hit
GGAGGAGAGGAAACCGCCTTGGCCGTACAGCAGAC	46919	0.2067048335686641	No Hit
GGACATCACTCACGCCTTGGCCGTACAGCAGACGG	46037	0.202819122807404	No Hit
GGACATCACCTACGCCTTGGCCGTACAGCAGACGG	45483	0.200378438270286	No Hit
GGACATCACAAACGCCTTGGCCGTACAGCAGACGG	45455	0.20025508237310313	No Hit
GGACATCACACGCCGCCTTGGCCGTACAGCAGACG	45303	0.19958543607411044	No Hit
GGACATCACATACGCCTTGGCCGTACAGCAGACGG	44726	0.19704342347859224	No Hit
GGACATCACAAACGCCGCCTTGGCCGTACAGCAGA	39629	0.17458824462355524	No Hit
GGACATCACTACGCCTTGGCCGTACAGCAGACGGA	38594	0.17002848199554596	No Hit
AAAGCATCGCGAAGGCCCGCCTTGGCCGTACAGCA	37644	0.1658431926268418	No Hit
GGACATCACTCACGCCGCCTTGGCCGTACAGCAGA	36619	0.16132748567639785	No Hit
GGAGGAGAAGAAGACCGCCTTGGCCGTACAGCAGA	34973	0.1540759211491483	No Hit
GGGGAAAGAAGACCGCCTTGGCCGTACAGCAGACG	34781	0.15323005213989438	No Hit
AAAGCATCGCGAAGACCCGCCTTGGCCGTACAGCA	34256	0.1509171290677158	No Hit
GGAGGAGAAGAAACGCCTTGGCCGTACAGCAGACG	30237	0.13321115225421887	No Hit
GAAAGCCGCCGTGGCGCAATCGCCTTGGCCGTACA	29813	0.13134319152544985	No Hit
GGACATCACTTCGCCTTGGCCGTACAGCAGACGGA	29299	0.1290787296985931	No Hit
GGACATCACTGACGCCGCCTTGGCCGTACAGCAGA	28253	0.12447050582526195	No Hit
GGACATCACTTAACGCCGCCTTGGCCGTACAGCAG	27831	0.12261135623200602	No Hit
GGACATCACTTACCGCCTTGGCCGTACAGCAGACG	27653	0.12182716517134355	No Hit
AAAGCATCGCGAAGGCCCACCGCCTTGGCCGTACA	27395	0.12069052869015862	No Hit
GGACATCACCTACGCCGCCTTGGCCGTACAGCAGA	26993	0.11891949045203327	No Hit
GGACATCACTTACACCGCCTTGGCCGTACAGCAGA	26670	0.11749649206667387	No Hit
GGACATCACTTACAGCCGCCTTGGCCGTACAGCAG	26347	0.11607349368131445	No Hit
GGACATCACTAACGCCTTGGCCGTACAGCAGACGG	24555	0.10817871626161142	No Hit
GGAGGAGAAGAACGCCTTGGCCGTACAGCAGACGG	23970	0.10560145912404095	No Hit
GGACATCACTTACCGCCGCCTTGGCCGTACAGCAG	23066	0.10161882587213722	No Hit
AAAGCATCGCGAAAGCCCGCCTTGGCCGTACAGCA	22825	0.1005570840428133	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGACATC	446485	28.628975	1
AAAGCAT	126875	28.500507	1
GACATCA	438555	28.314512	2
AAGCATC	128605	27.93407	2
ACATCAC	420390	27.933336	3
CATCACT	280245	27.840967	4
CATCACA	93725	27.721163	4
CTGCTGT	11940	27.698603	1
ATCACAA	23845	27.683498	5
AAGAAAC	61330	27.66364	8
ATCACTT	212370	27.518555	5
AGCATCG	130715	27.400253	3
ATCACAT	50550	27.399952	5
TCACTTA	193505	27.33403	6
ATCACTA	32865	27.274399	5
ATCACTC	31160	27.269821	5
TCACAAA	14320	27.260456	6
ACATCAA	9670	27.253513	3
CACAAAC	12805	27.216711	7
CACTTAC	178095	27.199207	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content