FastQCFastQC Report
Fri 30 Mar 2018
wt2_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt2_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46577734
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1147650.2463945541017517No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT965240.20723206500341987No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT926510.19891693314234654No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC881790.18931577908019312No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC860890.18482865654220104No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA796320.17096580954324658No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC737130.15825802088182306No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT728510.15640735120347415No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT707050.1517999995448469No Hit
AAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAGCCAATGAAATGGG684660.14699298166802188No Hit
ATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAG666730.1431435028591129No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA648440.13921673390122413No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT615680.13218333034406526No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG605980.13010079021877707No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT586930.12601085316859767No Hit
AAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATT581040.12474630045334538No Hit
CCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGATA544490.11689920338331615No Hit
AAAGAATTACAGCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAA542740.11652348738133117No Hit
CTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTA533050.11444309420462576No Hit
TATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTA529480.11367663356057639No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC506780.10880306027768546No Hit
CTCGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAG491000.1054151754140723No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC477250.10246312111276173No Hit
CAAAAACATCACCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAG470900.10109980876270193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)