FastQCFastQC Report
Fri 30 Mar 2018
790_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename790_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40472709
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT827410.20443652536330098No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA820050.2026180160067862No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC679800.16796503540200386No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC656790.16227972286213904No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC582340.1438846112327198No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC574520.14195244504142285No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG541250.13373209092576432No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT520400.12858047134922448No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA503390.1243776392630402No Hit
AAAGAATTACAGCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAA440810.10891536813115228No Hit
GCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAA434630.10738841326386134No Hit
CTCGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAG407350.1006480688011272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)