FastQCFastQC Report
Fri 30 Mar 2018
788_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename788_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57142089
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA1490250.26079725576711066No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT1201900.21033532743263902No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC1013220.17731588356876488No Hit
AAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAGCCAATGAAATGGG950340.16631173564550641No Hit
ATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAG869090.15209279450739016No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC793420.13885036649605162No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT769110.13459605930752724No Hit
TATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTA736340.1288612322171141No Hit
AAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATT714370.12501643053336745No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC711070.12443892277021933No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC708450.12398041660675024No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA706680.1236706624428799No Hit
CGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAA672950.11776783309409636No Hit
AAAGAATTACAGCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAA654300.11450403922054722No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT606720.10617742729006634No Hit
GAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTA603880.10568042060905404No Hit
GGAGGATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCG586570.10265112988781352No Hit
CTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTA585150.10240262654730736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)