FastQCFastQC Report
Fri 30 Mar 2018
788_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename788_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57142089
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC1822090.31887003641046446TruSeq Adapter, Index 13 (98% over 50bp)
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1620230.2835440615410473No Hit
CCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGAT1293780.22641454357750201No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT1263860.2211784731916259No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1141380.1997441850612077No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA1048610.18350921682264715No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG998270.17469959839935148No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA989240.1731193271565553No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG894360.15651510395428492No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT883420.1546005782182727No Hit
CTCTATTCTTAATTTACTACTAAATCCTCCTTAGTCCTTTAGTTTCATAA839790.14696522558004488No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA802820.1404953886092614No Hit
GCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACT727230.12726696078612035No Hit
GGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAG709490.12416241905331812No Hit
CTCCTTAGTCCTTTAGTTTCATAAAGGGTATAGTAATGTTCTTTTATAAG705970.12354641077262682No Hit
CTTTAGTTTCATAAAGGGTATAGTAATGTTCTTTTATAAGAAAATGTAGC690080.12076562339189245No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA676420.11837509125716422No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA672070.11761383102392353No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG665780.11651306622689275No Hit
GCTCAATTCAATTAAGCTCTCTATTCTTAATTTACTACTAAATCCTCCTT616650.10791520065008475No Hit
GAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACT610050.10676018512378854No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT574960.10061935257564698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)