FastQCFastQC Report
Fri 30 Mar 2018
784_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename784_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41801443
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA808330.19337370721867184No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT699750.16739852736662705No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC664380.15893709698012098No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC662140.15840123031159473No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG603450.14436104514382433No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC582910.13944733917439167No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA519150.12419427721669798No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT513180.12276609685459902No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC474810.11358698789417389No Hit
GCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAA455350.10893164621135207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)