FastQCFastQC Report
Fri 30 Mar 2018
784_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename784_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41801443
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2235740.5348475649512865TruSeq Adapter, Index 1 (100% over 50bp)
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1208250.28904504564591227No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA968970.2318030025901259No Hit
CCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGAT946820.22650414245268996No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG751500.17977848276673128No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA749660.17933830657472757No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA730500.17475473274929768No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT712580.17046779940108767No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA548000.13109595283588654No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT542230.12971561771204884No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG478510.11447212480200743No Hit
GCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACT428570.1025251688081677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)