FastQCFastQC Report
Fri 30 Mar 2018
759_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename759_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38522723
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA824830.2141151859903569No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT705060.1830244450788175No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC683200.17734987217803894No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC650220.16878869128747728No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC591800.1536236158591385No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC524850.13624426289906869No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT504440.13094609121997944No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA464170.12049252073899346No Hit
CTCGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAG435920.11315918659228737No Hit
AAAGAATTACAGCTAGAAACCCCGAAACCAAACGAGCTACCTAAAAACAA429990.11161983538910268No Hit
GCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAA404000.1048731679741331No Hit
GGAGGATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCG388670.10089369850620374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)