FastQCFastQC Report
Fri 30 Mar 2018
759_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename759_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38522723
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1028320.2669385546810904No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1019140.2645555455672227TruSeq Adapter, Index 3 (100% over 50bp)
CCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGAT918150.23833984944418388No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG911780.23668627993924518No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA830370.21555329824425964No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA779720.20240521419007687No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT638130.16565028385973649No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT576370.14961818768626506No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA570740.14815671259791266No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA538210.1397123458795994No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG446390.11587706299993382No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG444170.115300779750175No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA435280.11299305088064518No Hit
GCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACT399520.10371021799263774No Hit
CTCTATTCTTAATTTACTACTAAATCCTCCTTAGTCCTTTAGTTTCATAA397740.1032481530446329No Hit
GGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAG394420.10238632404048903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)