FastQCFastQC Report
Fri 30 Mar 2018
758_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename758_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26409088
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA625780.23695630837384465No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC521880.1976137911312954No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC462560.17515182652274852No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT455160.1723497608096122No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT428060.1620881417790724No Hit
ATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAG399900.15142514576800228No Hit
CTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTA373880.1415724768685689No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA367190.13903925800088213No Hit
CTCGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAG345970.1310041452396993No Hit
AAAGAATCAAACATAAAAACGTTAGGTCAAGGTGTAGCCAATGAAATGGG331680.12559312915311577No Hit
CTAAGTTATACCTCTTAGGGTTGGTAAATTTCGTGCCAGCCACCGCGGTC276970.10487677575234708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)