FastQCFastQC Report
Fri 30 Mar 2018
2058H_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2058H_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27030566
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCG1235100.4569271690426312No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA356020.13171015360906613No Hit
CGGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCC334890.12389307719268623No Hit
ATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCGGATCT313500.11597981337127752No Hit
CAAAAACATCACCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAG313180.11586142887278054No Hit
CGCGCGTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTCCCGCTCCCCGT307790.11386738997622174No Hit
CGGAACGATACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCG302790.11201763218720615No Hit
CCTCCACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGGCGCTCCGG290870.10760780961819297No Hit
CGGGAAGGCCCGGTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCAGG289420.10707137985937845No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC284450.10523272061709696No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA281680.1042079548019823No Hit
CCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTCCCG280800.10388239743111558No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC276420.10226200960793792No Hit
CCCGACCCCTCCACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGGC273400.1011447559033725No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC272360.10076000628325726No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC272090.10066011936265042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)