FastQCFastQC Report
Fri 30 Mar 2018
2058H_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2058H_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27030566
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT1357310.5021389489217503No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA1256880.46498471397158314No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1163210.4303313515521651TruSeq Adapter, Index 8 (100% over 50bp)
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG827140.30600173152127114No Hit
CCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGG513570.18999602154094739No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA467880.17309293486492291No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT461820.17085102842463604No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA439590.1626270052946727No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC418620.1548691211275413No Hit
CCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCG400080.14801021924587152No Hit
CCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTC393690.14564622879150957No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG383800.14198740788483674No Hit
CCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGAT376440.1392645644194058No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG371980.13761458047160388No Hit
CCCCGAGCCACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTC368640.13637894226854147No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG365840.13534307790669273No Hit
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAG359990.1331788612935445No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG356850.1320172134020427No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA336840.1246144827304023No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA329840.12202482182578048No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT318140.117696388599484No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG311880.11538049184763649No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT311270.11515482139737658No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGC310550.11488845627575833No Hit
CGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGC298940.11059331868966413No Hit
CGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGC297250.10996810055697688No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT294740.10903952214689104No Hit
GGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG294520.10895813280417435No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT290650.10752642027547629No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT288450.10671252684830942No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT286260.10590233293672059No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC283500.104881266637184No Hit
GGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAG276610.10233230040392052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)