FastQCFastQC Report
Fri 30 Mar 2018
2010H_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2010H_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377040
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC3346441.5654365618439223TruSeq Adapter, Index 11 (100% over 50bp)
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG438250.20500967393053482No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA414550.19392301272767418No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG383800.17953842066067144No Hit
CCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGAT336590.15745397866121785No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT326730.15284155336753824No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA310820.14539898882165164No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG286420.13398487349043647No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG258210.12078847211774875No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA254500.11905296523746974No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA242690.11352834630051682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)