FastQCFastQC Report
Fri 30 Mar 2018
2007H_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2007H_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27143712
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA487710.1796769726999756No Hit
ATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATC390250.14377178773485366No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC355450.13095113888623636No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT350210.12902067336995027No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA308570.11368010388557025No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA285120.10504090229074049No Hit
CCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTT275720.10157785346381512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)