FastQCFastQC Report
Fri 30 Mar 2018
2007H_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2007H_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27143712
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1979390.729225980588064TruSeq Adapter, Index 12 (100% over 50bp)
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT805060.29659171155367403No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT633580.2334168591237632No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA586010.21589162160282277No Hit
GGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAG582270.21451377026104607No Hit
CCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGAT499490.1840168360171225No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA488030.17979486372387093No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA458300.16884205078509526No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA436680.16087703848316695No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT377330.13901193764507963No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG375130.13820143685579922No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT369450.1361088711816571No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG362320.13348211180548925No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT306150.1127885530173618No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC291600.10742819552462095No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA290410.10698978827951018No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGT279180.10285255015968339No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT271990.10020368621653516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)